Skip to main content
. 2017 Sep 11;18(11):2051–2066. doi: 10.15252/embr.201744137

Figure EV3. hBAR stabilizes F‐actin.

Figure EV3

  1. Effect of hBAR on the rate of actin depolymerization. Dilution‐induced depolymerization of pyrene‐labeled actin in the presence or absence of hBAR at indicated molar ratios was measured following pyrene fluorescence intensity over time. Dotted lines indicate the one‐phase exponential decay fitting.
  2. Calculated half‐time of actin depolymerization from (A) after one‐phase exponential decay fitting. Statistically significant differences are indicated (one‐way ANOVA, ****P < 0.0001, n = 3). The error bars represent mean ± SD.
  3. Effects of cofilin and hBAR on dilution‐induced depolymerization of F‐actin. Spontaneous disassembly of pyrene‐labeled F‐actin in the presence or absence of hBin1 (isoform 1) and cofilin was followed, as measured by pyrene fluorescence. Dotted lines indicate the one‐phase exponential decay fitting.
  4. Calculated half‐time of actin depolymerization from (C) after one‐phase exponential decay fitting. Statistically significant differences are indicated (one‐way ANOVA, ****P < 0.0001, n = 3). The error bars represent mean ± SD.