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. 2017 Sep 11;18(11):2051–2066. doi: 10.15252/embr.201744137

Figure EV5. Biochemical validation of dBin1 downregulation in fly brains.

Figure EV5

  1. Exponential fitting of depolymerization of tau‐induced actin bundles. Actin depolymerization data from Fig 5G were fit to a single exponential decay. The displayed fits are the basis for the half‐time calculation from Fig 5H.
  2. Phalloidin staining of whole‐mount brain (22 days old) dissected from tauR406W transgenic flies and control flies. The zoom‐in images represent one single section in the middle of the central body of the fly brain, and the white arrows highlight actin rods. Scale bar of brain overview: 100 μm and zoom‐in: 25 μm.
  3. Knockdown efficiency of different dBin1 RNAi lines. The dBin1 lines were expressed pan‐neuronally under the Elav promoter, and fly heads were subjected to protein extraction 10 days post‐eclosion. Samples were resolved by SDS–PAGE and immunoblotted.