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. 2017 Sep 8;18(11):2067–2078. doi: 10.15252/embr.201744228

Figure 1. Sustained cold stress activates the ASK1‐p38 pathway.

Figure 1

  • A
    Immunoblots of endogenous phospho‐ASK, phospho‐p38, and phospho‐JNK signals in the lysate from A549 cells treated with cold stress for the indicated time.
  • B
    The temperature transition of culture medium placed on ice. Mean ± SEM, N = 3.
  • C
    Immunoblots of endogenous MAPK signals in A549 lysate treated with cold stress on ice for the indicated time. A star indicates non‐specific signal.
  • D, E
    Immunoblots of endogenous phospho‐ASK and phospho‐p38 signals in siRNA‐treated A549 (D) or in HEK293A cells (E). Cold stress on ice was applied for the indicated time.
  • F
    Endogenous thioredoxin was immunoprecipitated from A549 lysate with an anti‐thioredoxin antibody after 4 h of cold stress on ice, and the immune complex was examined by Western blot analysis.

Source data are available online for this figure.