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. 2017 Sep 8;18(11):2067–2078. doi: 10.15252/embr.201744228

Figure 5. The ASK1‐p38 pathway is activated through the course of erastin‐induced ferroptosis.

Figure 5

  1. Immunoblot of endogenous phospho‐ASK and phospho‐p38 signals in the lysate of A549 cells treated with 10 μM erastin for 16 or 18 h.
  2. Immunoblot of endogenous phospho‐ASK and phospho‐p38 signals in A549 cells after 16 h of treatment with 10 μM erastin in the presence or absence of 1 μM ferrostatin‐1. The pretreatment was for 30 min prior to erastin administration.
  3. The effect of siRNA‐mediated knockdown of ASK1 was assessed by Western blot analysis. A549 cells were treated with 5 μM erastin for 16 h.

Source data are available online for this figure.