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. 2017 Nov 2;36(21):3212–3231. doi: 10.15252/embj.201796585

Figure EV3. Actin dynamics is required for DNA replication.

Figure EV3

  • A
    Nuclei were allowed to form for 30 min before drugs (CytD, CD; SMIFH2, FH; latrunculin A, LA; jasplakinolide, Jpk; CytD and latrunculin A, CD/LA; SMIFH2 and latrunculin A, FH/LA) or Arp2/3 recombinant protein (in combination with VCA domain of WASP) was added, then purified at 45 min. Soluble and insoluble nuclear fractions were blotted for actin. Equal number of nuclei was used in each condition.
  • B–F
    DNA replication assessed in control extract, or extracts supplemented with CytD (CD), with or without latrunculin A (LA) (B); CytD (CD) with or without cofilin (C); CytD (CD), gelsolin, CytD and gelsolin (CD + Gel), jasplakinolide (Jspk), or CytD and jasplakinolide (CD + Jspk) (D); recombinant MICAL2 protein (E), or formin inhibitor 2.4 (F). Each panel is representative of multiple experiments: (B) 5 experiments; (C–F) 2 experiments.
  • G
    DNA replication analysed in control extract, or extracts supplemented with CytD, SMIFH2 or PA that was added at the time points indicated. Representative of two independent experiments.

Source data are available online for this figure.