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. 2017 Nov 2;36(21):3212–3231. doi: 10.15252/embj.201796585

Figure 8. Nuclear formin activity controls chromatin loading of PCNA and CDKs.

Figure 8

  • A–C
    Scheme: Nuclear transfer from the first extract, where active nuclear export (leptomycin B, LB) and CDK (PA) were inhibited, into the second extract, either control or treated with SMIFH2, containing leptomycin B or not. (B) DNA replication was assessed in the second extract. (C) Total nuclear and chromatin‐associated replication factors in nuclei isolated at 60 min were analysed by Western blotting. PCNA and PSTAIR antibodies were sequentially used to blot the same area of membrane.
  • D
    Chromosomal DNA replication in NPE determined by 33P‐dCTP incorporation assay in control conditions (Ctl) or in the presence of SMIFH2; mean ± SEM of four independent experiments.
  • E
    Time course of chromatin loading of indicated replication factors in NPE, in control (Ctl) or SMIFH2‐treated extracts (500 μM); SE, short exposure; LE, long exposure.

Source data are available online for this figure.