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. 2017 Oct 13;36(21):3250–3267. doi: 10.15252/embj.201796794

Figure 3. Down‐regulation of PKAc1 leads to premature egress and “restless invasion”.

Figure 3

  • A
    A drop in PKAc1‐Ty‐iKD expression is detected as early as 24 h upon ATc treatment with almost no protein detectable after 36 h.
  • B
    Conditional down‐regulation of PKAc1‐Ty‐iKD leads to the absence of plaque formation.
  • C, D
    Down‐regulation of PKAc1‐Ty‐iKD upon ATc treatment leads to increased parasite dispersion after 40 h and premature egress (data are from three independent biological replicates).
  • E
    Prematurely egressed PKAc1‐Ty‐iKD parasites after 24 h of ATc treatment invade and exit fully lysing the monolayer of HFF cells, while the non‐treated parasites invade and initiate a new lytic cycle.
Data information: In (D), data are presented as mean ± SD. Scale bars = 1 mm (B), 20 μm (C), 2.5 mm (E). Source data are available online for this figure.