Figure 1. LRBA expression in the organ of Corti.

• A, A′
  Representative immunofluorescence images from a WT p15 organ of Corti cryosection stained for LRBA (magenta), the hair cell marker parvalbumin (PV; green), and the spiral ganglion neuronal fiber marker neurofilament 200 (NF200; orange); nuclei are counterstained with DAPI. LRBA immunoreactivity could be detected in hair cells, multiple types of supporting cells, the marginal cells of the stria vascularis (SV), and Reissner's membrane (RM). (A′) Inset shows a higher magnification view of the sensory epithelium, but omitting the DAPI staining for clarity. Scale bars: 50 μm, in inset: 10 μm. IHC, inner hair cells; OHC, outer hair cells; TM, tectorial membrane.
• B, B′
  Representative confocal maximum projections from acutely dissected (B) WT and (B′) Lrba‐KO p15 apical turn whole‐mount organs of Corti immunostained for LRBA (magenta), NF200 (blue), and PV (green). Scale bars: 100 μm.