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. 2017 Sep 11;18(11):2015–2029. doi: 10.15252/embr.201643689

Figure 4. LRBA localizes to the kinocilium basal body and progressively encircles the cuticular plate.

Figure 4

  • A
    Schematic illustration of LRBA protein domains and the LRBA‐antibody binding sequence (*LRBA‐abs). LamG, laminin G domain; ARM, Armadillo/β‐catenin‐like repeats; WD40, WD40 repeat; DUF, domain of unknown function; PH, pleckstrin homology domain; BEACH, BEACH domain.
  • B–E″
    Developmental range (B–B″) p0, (C–C″) p3, (D–D″) p7, (E–E″) p10 showing LRBA clustering at a central position beneath the hair bundle in both, IHCs and OHCs. Samples were stained with specific antibodies against β2‐spectrin (green; cuticular plates) and LRBA (magenta) as well as fluorophore‐conjugated phalloidin (blue; stereocilia). Close‐ups of individual LRBA signals for (B′, C′, D′, E′) OHCs and (B″, C″, D″, E″) IHCs are presented in an intensity‐coded lookup table for clarity and show two adjacent hair cells surrounded by supporting cells as indicated in the dashed boxes in (B, C, D, E) (please also refer to Appendix Fig S1C and D for Lrba‐KO stainings, where all LRBA signal from hair cells and supporting cells is entirely lost). Scale bars: 5 μm.
  • F
    Schematic yz overview of the LRBA localization as derived from stainings with the indicated (F′) cuticular plate (CP) and (F″) kinocilium (KC) markers. Panels (F′, F″) show representative yz views through single p7 IHC cuticular plates at a central plane through the kinociliar pore. LRBA immunofluorescence appears to line the pore. Scale bars: 1 μm.