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. 2017 Sep 11;18(11):2015–2029. doi: 10.15252/embr.201643689

Figure EV5. No major loss of cochlear hair cells can be observed in 29‐week‐old Lrba mutants and the afferent innervation pattern is maintained; moreover, vestibular hair cells do not show a prominent hair bundle phenotype.

Figure EV5

  • A, A′
    Representative confocal maximum projections of 29‐week‐old (A) wild‐type and (A′) Lrba‐KO organs of Corti stained with a specific antibody against the exocytic protein otoferlin (magenta). Actin‐rich structures were counterstained with fluorophore‐conjugated phalloidin. Scale bars: 10 μm.
  • B, B′
    IHC afferent innervation patterns in (B) wild‐type and (B′) Lrba mutants are maintained after 29 weeks. Specimen were stained with specific antibodies against NF200 (green; SGN afferent fibers) and the IHC‐specific marker Vglut3 (magenta). Scale bars: 10 μm.
  • C–C″
    Utricular hair cells also express LRBA at the fonticulus, but do not show any obvious hair bundle deficits at an age where cochlear hair bundles are degenerated. (C) Detail of a single utricular hair bundle illustrating LRBA localization at the fonticulus. Overviews of (C′) wild‐type and (C″) Lrba‐KO hair bundles from p22 utricles. Scale bars: 5 μm in (C); 10 μm in (C′ and C″).