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A
Schematic diagram of a germarium showing the somatic cells (blue) and the germline cells (green). The spectrosomes and fusomes are shown in orange. The different regions of the germarium are indicated. Region 1: dividing cysts; region 2: selection of the oocyte; region 3: egg chamber with posteriorly localized oocyte. GSCs, germline stem cells; CB, cystoblast.
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B–E
Immunostaining of germaria from 7‐day‐old females with anti‐Vasa (green, B–D) or anti‐GFP (green, E), and anti‐Hts (red). DAPI (blue) was used to visualize DNA. (B) aub
HN2/+ was used as a control. (C, D) Examples of aub
HN2/QC42 germ cell loss and tumor, respectively. (E) Phenotypic rescue of aub
HN2/QC42 with UASp‐GFP‐Aub expressed using nos‐Gal4. White arrowheads indicate GSCs; the white arrow indicates GSC loss.
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F
Quantification of mutant germaria with 0–1 GSC, or with GSC tumors shown in (B–E). The number of scored germaria (n) is indicated on the right graph. Error bars represent standard deviation. ***P‐value < 0.001, ns, non‐significant, using the χ2 test.
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G–H’
Germaria containing control (G, G’) or aub
HN2 mutant (H, H’) clonal GSCs stained with anti‐GFP (green) and anti‐Hts (red), 14 days after clone induction. DAPI (blue) was used to stain DNA. Clonal cells are marked by the lack of GFP. Clonal GSCs and cysts are outlined with dashed line. White arrowheads show clonal GSCs in the control. aub mutant clonal GSCs have been lost (H, H’).
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I
Quantification of germaria containing at least one clonal GSC at 7, 14, and 21 days after clonal induction. 50 to 219 germaria were analyzed per condition. Error bars represent standard deviation.
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J
Division rate of wild‐type and aub
HN2 clonal GSCs. The number of scored germaria (n) is indicated. Error bars represent standard deviation. ***P‐value < 0.001 using the two‐tailed Student's t‐test.
Data information: Scale bars: 10 μm in (B–E) and (G–H’).
