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. 2017 Oct 13;36(21):3194–3211. doi: 10.15252/embj.201797259

Figure EV1. GSC loss in aub mutant is independent of apoptosis and of Bam expression.

Figure EV1

  • A, A’
    aub HN2 clonal GSCs do not express Aub protein. Immunostaining of mosaic germaria with anti‐GFP (green) and anti‐Aub (red) 7 days after clonal induction. DAPI (blue) was used to visualize DNA. The white and yellow arrowheads indicate the control aub HN2/+ and the aub HN2 clonal GSCs, respectively.
  • B–C’
    aub mutant GSCs do not die by apoptosis. Immunostaining of control aub HN2/+ (B, B’) and mutant aub HN2/QC42 (C, C’) germaria with anti‐cleaved Caspase3 (green) and anti‐Hts (red). DAPI (blue) was used to visualize DNA. GSCs are outlined.
  • D
    Quantification of germaria shown in (B–C’) with cleaved Caspase3‐positive GSCs in 7‐, 14‐ and 21‐day‐old females. The percentages are not statistically different using the χ2 test.
  • E–F’
    aub mutant GSCs do not express Bam. Immunostaining of control aub HN2/+ (E, E’) and mutant aub HN2/QC42 (F, F’) germaria with anti‐Bam (green) and anti‐Hts (red). DAPI (blue) was used to visualize DNA. GSCs are outlined.
  • G
    Quantification of mutant germaria with 0–1 GSC shown in Fig 2A and B, showing that the GSC loss remains high in chk2 aub double mutant. The genotypes are indicated at the top. The number of scored germaria (n) is indicated in Fig 2C.