Figure 2. The role of Aub in GSC self‐renewal is partially independent of Chk2 and requires its loading with piRNAs.

• A, B
  Immunostaining of germaria with anti‐Vasa (green) and anti‐Hts (red). DAPI (blue) was used to visualize DNA. Examples of mnk ^P6 and mnk ^P6 aub ^HN2/QC42 germaria are shown. White arrowheads indicate GSCs; the white arrow indicates GSC loss.
• C
  Quantification of the number of GSCs per non‐tumorous germarium, and of germaria with GSC tumors, in the indicated genotypes. The number of scored germaria (n) is indicated. Error bars represent standard deviation. The two‐tailed Student's t‐test and the χ² test were used in the left and right panels, respectively. ***P‐value < 0.001, **P‐value < 0.01, *P‐value < 0.05.
• D–F
  Immunostaining of germaria from 7‐day‐old females with anti‐Vasa or anti‐GFP (green) and anti‐Hts (red). DAPI (blue) was used to visualize DNA. aub ^HN2/QC42; nos‐Gal4/+ was used as a negative control. Examples of rescue in aub ^HN2/QC42; nos‐Gal4/UASp‐GFP‐Aub germarium (E), and of lack of rescue in aub ^HN2/QC42; nos‐Gal4/UASp‐GFP‐Aub ^AA germarium (F). White arrowheads indicate GSCs; white arrows indicate GSC loss in (D) and germ cell loss in (F).
• G
  Quantification of mutant germaria with 0–1 GSC shown in (D–F). The number of scored germaria (n) is indicated. Error bars represent standard deviation. ***P‐value < 0.001, ns, non‐significant, using the χ² test.

Data information: Scale bar: 10 μm in (A, B) and (D–F).