Figure EV1. SIRT1 KO mESCs have altered methionine metabolism and SAM production when cultured in the M10 maintenance medium.

1. Significant enrichment of the methionine metabolism pathway in SIRT1 KO versus WT mESCs cultured in the M10 medium. WT and SIRT1 KO mESCs cultured in the complete medium were harvested and subjected to global metabolomic analysis by Metabolon. The fold enrichment of amino acid metabolism pathways was analyzed by Cytoscape 2.8.3 (n = 5 independent experiments). Yellow, P = 0.03.
2. Significant enrichment of the methionine metabolism pathway in SIRT1 KO versus WT mESCs cultured in the complete medium. WT and SIRT1 KO mESCs were cultured and analyzed by Metabolon as in (A). The fold enrichment of amino acid metabolism pathways was analyzed by the metabolite set enrichment analysis (MSEA) of MetaboAnalyst 3.0 (n = 5 independent experiments).
3. Schematic of one‐carbon metabolism and glutathione homeostasis in SIRT1 KO mESCs relative to WT mESCs in the complete M10 medium. WT and SIRT1 KO mESCs cultured as in (A). The log ratios of the relative abundance of metabolites in indicated pathways in KO/WT mESCs were presented by color scale (n = 5 independent experiments). All colored metabolites were significantly changed in KO mESCs compared to WT mESCs with P < 0.05.