Figure 4.

Aspirin and apatinib inhibit tumorigenesis of GC cells. (A and B) The IC₅₀ values of aspirin and apatinib were detected using a cell proliferation assay kit. (C) The proliferation of aspirin and/or apatinib-treated MKN45 cells was determined after 0, 24, 48 and 72 h. Experiments were performed in triplicate for each group. (D and E) Migration and invasion of MKN45 cells following treatment with aspirin and/or apatinib. (F) Representative micrographs and (G) quantification of crystal violet-stained cell colonies. The control cells were treated with dimethyl sulfoxide. *P<0.05, **P<0.01, aspirin or apatinib treated group vs. the control group. GC, gastric cancer; IC₅₀, 50% inhibitory concentration; SD, standard deviation.