Figure 1.

(A) miR-193a-3p is downregulated in prostate cancer cell lines. Reverse transcription-quantitative polymerase chain reaction, using U6 for normalization, was utilized to assess the expression levels of miR-193a-3p in DU-145 and PC3 prostate cancer cells and RWPE non-cancerous prostate cells, revealing decreased expression levels in the cancerous cell lines compared with the non-cancerous cells (*P<0.05 and ***P<0.001). (B) Cell viability/cell growth assays revealed that the relative cell viabilities (with cell viability at 0 nM regarded as 1.0) of the miR-193a-3p-transfected groups of DU-145 and PC3 cells were lower, compared with the NC-transfected groups (*P<0.05, **P<0.01 and ***P<0.001 vs. NC). An optimal concentration for miR-193a-3p was identified between 25 and 50 nM for DU-145 and PC3 cells. (C) Colony formation assays indicated that the colony formation rate was lower for miR-193a-3p-transfected groups compared with NC-transfected groups (**P<0.01 and ***P<0.001); representative wells are shown. (D) Cell cycle profiles in DU-145 and PC3 cells are shown. Overexpression of miR-193a-3p induced a significant G₁-phase arrest in DU-145 and PC3 cells (**P<0.01 and ***P<0.001 vs. NC). Representative histograms are depicted and data are presented as the average of repeated experiments. miR, microRNA; NC, negative control.