Table I.
Oligonucleotide and primer sequences.
| Name | Sequence (5′-3′)a |
|---|---|
| miR-193a-3p mimic (sense) | AACUGGCCUACAAAGUCCCAGU |
| NC | ACUACUGAGUGACAGUAGA |
| siCCND1 (sense) | GGAGAACAAACAGAUCAUC |
| U6-F | TGCGGGTGCTCGCTTCGGCAGC |
| miR-193-3p-F | ACTGGCCTACAAAGTCCCAGT |
| CCND1-F | GCTGCGAAGTGGAAACCATC |
| CCND1-R | CCTCCTTCTGCACACATTTGAA |
| GAPDH-F | ACAACTTTGGTATCGTGGAAGG |
| GAPDH-R | GCCATCACGCCACAGTTTC |
| CCND1-UTR-F | ATTGCAGAGGATGTTCATAAGGCCAGTATGATTTATAAATGCAAT |
| CCND1-UTR-R | ATTGCATTTATAAATCATACTGGCCTTATGAACATCCTCTGCAAT |
| CCND1-Mut-F | ATTGCAGAGGATGTTCATAACCGGTCAATGATTTATAAATGCAAT |
| CCND1-Mut-R | ATTGCATTTATAAATCATTGACCGGTTATGAACATCCTCTGCAAT |
a
Target sites are in italic and bold type; mutated target sites are in bold type and underlined. miR, microRNA; NC, negative control; si, small interfering RNA; CCND1, cyclin D1; F, forward primer; R, reverse primer; UTR, untranslated region; Mut, mutant.