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. 2017 Sep 1;31(17):1732–1737. doi: 10.1101/gad.303768.117

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© 2017 Sze et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — Set1A^ΔSET mutants exhibit defective EB differentiation. (A) Wild-type and Set1A^ΔSET ESCs were induced to form EBs. Emboss contrast images were taken at day 6 of EB formation. Bar, 100 µm. (B) Box plot contrasting the size (in square micrometers) of wild-type day 6 EBs to that of Set1A^ΔSET EBs. The number of EBs measured per sample per replicate is indicated. P-values were calculated using the Student's t-test. (C) MA plot of gene expression changes between undifferentiated wild-type ESCs and wild-type day 6 EBs. (Yellow) Up-regulated genes during differentiation; (blue) genes down-regulated in Set1A^ΔSET EBs not pertinent to differentiation; (green) genes down-regulated in Set1A^ΔSET EBs but activated during normal differentiation. Colors correspond to the categories illustrated in Supplemental Figure S3B. (D) Two genome browser track examples of genes Bmp5 and Epor down-regulated in Set1A^ΔSET EBs compared with wild-type EBs. (E) Example genome tracks of decreased H3K4me3 occupancy in Set1A^ΔSET EBs compared with wild-type EBs for the two genes shown in D.