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. 2017 Sep 1;31(17):1770–1783. doi: 10.1101/gad.305482.117

Figure 1.

Figure 1.

Identification of activators and inhibitors of 5F-mediated cardiac reprogramming from a human ORF cDNA screen. (A) Schematic diagram of the human ORF cDNA library screen strategy for cardiac reprogramming in adult TTFs. (B) Venn diagram showing the number of activators identified from the screen. Genes with Z-scores of αMHC-GFP or cTnT expression ≥2 were defined as activators. Twenty-five genes induced αMHC-GFP expression only, 35 genes induced cTnT expression only, and 11 genes induced expression of both markers. (C) Venn diagram showing the number of inhibitors identified from the screen. Genes with Z-scores of αMHC-GFP or cTnT expression −2 or lower were defined as inhibitors. One-hundred-twenty-one genes repressed αMHC expression only, 41 genes repressed cTnT expression only, and 33 genes repressed both αMHC-GFP and cTnT expression. (D) Representative immunocytochemistry images of TTFs from adult αMHC-GFP transgenic mice treated with 5F and either empty virus or viruses encoding activators (ZNF281 or PHF7) and inhibitors (FOXA3 or SOX9). Cells were fixed and stained for αMHC-GFP (green), cTnT (red), and Hoechst (blue) 9 d after infection. Bars, 2 mm. (E) Pathways enriched in activators (n = 49) and inhibitors (n = 129), respectively, by DAVID pathway analysis.