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. 2017 Sep 1;31(17):1770–1783. doi: 10.1101/gad.305482.117

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© 2017 Zhou et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 2. — ZNF281 enhances cardiac reprogramming of adult fibroblasts. (A) Immunocytochemistry images of adult αMHC-GFP transgenic TTFs 7 d after infection with empty, ZNF281, 5F, or 6F retroviruses show that ZNF281 enhances expression of cardiac markers with 5F. (Green) αMHC-GFP; (red) cTnT; (blue) Hoechst. Bars, 500 µm. (B,C) Representative flow cytometry plot (B) and analyses (C) of αMHC-GFP⁺ and cTnT⁺ TTFs 7 d after infection with empty, 5F, or 6F. (D) TTFs were infected with empty, 5F plus empty, and ZNF281 for 7 d. Transcript levels of cardiac marker genes (Myh6 and Actc1) and fibroblast marker genes (Col1a2 and Sox9) were determined by qPCR. (*) P < 0.05.