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. 2017 Sep 1;31(17):1770–1783. doi: 10.1101/gad.305482.117

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© 2017 Zhou et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 4. — ZNF281 interacts with GATA4 to synergistically activate cardiac genes. (A) Coimmunoprecipitation assays were performed using HEK293 cells transfected with equal amounts of plasmid DNA encoding Myc-tagged GATA4, HAND2, MEF2C, or TBX5 and/or Flag-tagged ZNF281. (IP) Immunoprecipitation; (IB) immunoblot. (B) Heat maps showing ChIP-seq (chromatin immunoprecipitation [ChIP] followed by high-throughput sequencing) data for H3K27ac in adult mouse hearts and ZNF281 and GATA4 binding in reprogrammed TTFs at ±5 kb around the peak center. ChIP-seq experiments were performed using TTFs infected with 6F for 2 d. (C) ZNF281-binding motif enriched within ZNF281-binding peaks. (D) GATA4-binding motif enriched within GATA4-binding peaks. (E) Venn diagram showing the number of overlapping peaks between heart H3K27ac, ZNF281, and GATA4. (F) Total number of peaks identified by ChIP-seq.