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. 2017 Sep 22;18(10):2035. doi: 10.3390/ijms18102035

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Figure A2 — Titer and specificity analysis of the pAb against recombinant lamprey Vav3. (a) Determination of the titer of anti-recombinant lamprey Vav3 pAb by ELISA method. The anti-recombinant lamprey Vav3 pAb and negative control (rabbit IgG) were serially diluted to 64,000-fold in the same way, and the signal of each dilution was detected by three independent experiments; (b) the specificity of the anti-recombinant lamprey Vav3 pAb was confirmed by Western blot method. M: molecular marker; lane 1: the purified recombinant lamprey Vav3 (5 ng); lane 2: the purified recombinant lamprey Vav3 (15 ng) (n = 3).