Table 2.
Primers and qPCR conditions.
Gene | Primer Sequence | qPCR Cycling Conditions | Number of Cycles | Product Size (bp) |
---|---|---|---|---|
ALDH1A1 | Fwd: 5′-CGT TGG TTA TGC TCA TTT GGA A-3′ Rev: 5′-TGA TCA ACT TGC CAA CCT CTG T-3′ |
60 s 55 °C 60 s 72 °C 60 s 95 °C |
45 | 22 bp |
ALDH1A3 | Fwd: 5′-ATG TGG GAA AAC CCC CTG TG-3′ Rev: 5′-GAA TGG TCC CAC CTT CAC CT-3′ |
60 s 57 °C 60 s 72 °C 60 s 95 °C |
45 | 20 bp |
GAPDH | Fwd: 5′-CAT GTT CGT CAT GGG TGT GAA CCA-3′ Rev: 5′-ATG GCA TGG ACT GTG GTC ATG AGT -3′ |
45 s 60 °C 45 s 72 °C 60 s 95 °C |
40 | 24 bp |