Figure 3.

Effects of tanshinone IIA on phosphorylation and expression levels of STAT-3/5 during 3T3-L1 preadipocyte differentiation. (A) 3T3-L1 preadipocytes were differentiated with induction medium containing MDI, insulin and FBS in the presence or absence of tanshinone IIA, and harvested at D2, D5, and D8, respectively. Total cellular protein at the indicated time point were extracted and analyzed by Western blot analysis; (B) Western blot analysis in triplicate experiments on D8; (C) Western blot analysis in triplicate experiments on D2; (D,E) the densitometry data of (B,C), respectively.