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. 2017 Sep 27;18(10):2065. doi: 10.3390/ijms18102065

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of tanshinone IIA on lipolysis and hormone-sensitive lipase (HSL) phosphorylation in differentiated 3T3-L1 cells. (A) Differentiated 3T3-L1 cells were serum-starved for 2 h and treated with tanshinone IIA or isoproterenol (ISO) at the indicated concentration for additional 24 h. Glycerol contents were measured in triplicates. Data are mean ± SE of three independent experiments. * p < 0.05 vs. control; (B) Differentiated 3T3-L1 cells were serum-starved for 2 h and treated with tanshinone IIA or ISO at the indicated concentration for additional 24 h. Cellular protein was extracted and analyzed by Western blot analysis.