Figure 5.

Image section of a cell nucleus of a breast cancer cell line (SKBr3) after staining of heterochromatin (green) and γH2AX foci (red) by fluorescent specific antibodies. The cells were irradiated by 1 Gy photon-radiation and the images were acquired 60 minutes after irradiation during activated repair. (A) wide-field fluorescence image, scale bar: 2 µm ; (B) localization microscopy image of the same image section, scale bar: 2 µm; (C) enlarged inserts of A separated in the color channels red and green, image size 2 µm × 2 µm; (D) enlarged inserts of B separated in the color channels red and green, image size 2 µm × 2 µm.