Table 4.

Six factors worth consideration to develop the ideal pre-treatment process.

No#	Noteworthy Factors
1	The pretreated sample must be compatible with conventional or up-to-date detection methods.
2	It should be possible to shorten the total experiment time compared to the existing pre-enrichment procedure.
3	There should be little influence on the microorganisms’ growth.
4	The level of inhibitors present in the food and/or food homogenates should be minimized after pretreatment.
5	The method should ensure the effective isolation and recovery of target microorganisms from food surfaces or interiors.
6	The sample weight or buffer amount specified by regulatory organizations (e.g., ISO, USDA, and FDA) should be followed when performing the pretreatment.