| Symbol |
Name |
Unit |
|
EF
|
Fluorescence quantum distribution |
nm−1
|
|
gLED
|
Normalized spectral distribution of excitation LED |
|
|
Iexc
|
Excitation light intensity |
W cm−2
|
|
Isat
|
Excitation saturation intensity |
W cm−2
|
| l |
Length |
cm |
|
lexc
|
Sample length in excitation direction |
cm |
| n |
Refractive index |
|
| N |
Number density |
cm−3
|
|
Pexc
|
Light excitation power |
W |
| rpm |
Rotations per minute |
min−1
|
| t |
Time |
s, min, or h |
| T |
Transmission |
|
|
tm
|
Protein melting time |
h or d |
|
texc
|
Time of sample excitation |
s |
|
tres
|
Time resolution |
s |
| wexc
|
Excitation energy density |
J cm−2
|
| α |
Attenuation coefficient |
cm−1
|
| αa
|
Absorption coefficient |
cm−1
|
| αs
|
Scattering coefficient |
cm−1
|
| γ |
Scattering exponential factor |
|
| ϑ |
Temperature |
°C |
|
ϑm
|
Apparent melting temperature |
°C |
| ĸla1
|
Limiting fraction of excited CaRhda* converted to CaRhla1 at high excitation intensity |
|
| ĸla2
|
Limiting fraction of excited CaRhda* converted to CaRhla2 at high excitation intensity |
|
| λ |
Wavelength |
nm |
|
λF,exc
|
Fluorescence excitation wavelength |
nm |
|
λF,max
|
Wavelength position of maximum fluorescence emission |
nm |
|
Mean fluorescence wavelength |
nm |
| ν |
Frequency |
Hz |
|
Wavenumber |
cm−1
|
|
Fluorescence Stokes shift |
cm−1
|
|
Fluorescence spectral half-width (FWHM) |
cm−1
|
|
Absorption spectral half-width (FWHM) of S0–S1 transition |
cm−1
|
| σa
|
Absorption cross-section |
cm2
|
|
Absorption band cross-section strength |
cm2
|
|
τF
|
Fluorescence lifetime |
ps |
|
τrad
|
Radiative lifetime |
ns |
|
τrec
|
Recovery time constant |
s |
| Φcis
|
Quantum yield of trans-cis isomerization |
|
| Φd
|
Quantum yield of photo-degradation |
|
| ΦF
|
Fluorescence quantum yield |
|
| Φtrans
|
Quantum yield of trans back isomerization |
|
| ∆nph,abs
|
Increment of absorbed excitation photons |
cm−2
|
| N |
Increment of length-integrated number density |
cm−2
|