Figure 4.

Protein carbonylation in rat kidney slice homogenates and plasma proteins. Representative Western blot with anti-DNP antibody for PCOs of kidney slice homogenates (A) and plasma proteins (B) of adult male Sprague–Dawley rats treated with a single i.t. instillation of AgNPs or AgNO₃. In both (A) and (B): 1, control (7 days); 2, AgNO₃ (7 days); 3, AgNPs (7 days); 4, control 28 days); 5, AgNO₃ (28 days); 6, AgNPs (28 days). (C) human serum albumin (HSA) in the reduced form (−) and in the carbonylated/oxidized form (+). Protein molecular weight markers are shown on the left side of each panel. Although there were no differences between controls and treated samples, the most carbonylated proteins are highlighted by an asterisk (*) in kidney and plasma samples.