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. 2017 Oct 11;18(10):2121. doi: 10.3390/ijms18102121

Figure 1.

Figure 1

Effect of DOXO on calcium homeostasis. Mice received a single administration (1th group), two administrations (2nd group) or three administrations (3rd group) of DOXO (2 or 10 mg/kg; i.p.) and primary cardiomyocytes were isolated by enzymatic digestion. Intracellular calcium content in cells suspension was evaluated by using ionomycin (1 μM) (A); reticulum calcium content was evaluated by means of thapsygargin (100 nM) (B) and mitochondrial calcium content was evaluated by using FCCP (50 nM) (C). Results were expressed as mean ± S.E.M. of delta (δ) increase of FURA-2 AM ratio fluorescence (340/380 nm) from at least three independent experiments each performed in duplicate. Data were analyzed by Student’s t-test. * p < 0.05, ** p < 0.005 and *** p < 0.001 vs. control. Effect of DOXO on SERCA II (D) and PLB (E) expression. Mice received a single administration (first group), two administrations (second group), or three administrations (third group) of DOXO (2 mg/kg or 10 mg/kg; i.p.) and SERCA II and PLB expressions were detected by Western blot analysis into tissue homogenates from mice; GAPDH protein expression was used as loading control. Values were expressed as mean ± S.E.M. from at least three independent experiments each performed in duplicate. Data were analyzed by Student’s t-test. *p < 0.05 vs. control.