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Primers used in experiments.
| Primers | Sequences of Primers (5′ to 3′) | Purpose |
|---|---|---|
| F1 | GTCTCTACGCCTGCTATCGC | RT-qPCR |
| R1 | GACGCATCCACCTCAGTTTGT | RT-qPCR |
| β-actin-F2 | GGGCCGGACTCGTCATATT | RT-qPCR |
| β-actin-R2 | ATCACAGCCCTCGCTCGCTCCAT | RT-qPCR |
| F3 | CGGAATTC*AAAGACAGCATGCACGC (EcoR I) | Protein expression |
| R3 | CCCAAGCTTTTCCTTGGGTTTGGGAT (Hind III) | Protein expression |
* The underline represented restriction enzyme cutting site.
