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. 2017 Oct 14;18(10):2146. doi: 10.3390/ijms18102146

Figure 6.

Figure 6

Effects of Taurine treatment on expression of calpains and autophagy-relative proteins induced by EBSS in ARPE-19 cells. (A) Cells were treated with 0.1–60 mmol/L taurine for 24 h. Cell viability was evaluated via CCK-8 assays; (B–D) Western blot analysis was performed to detect the expression levels of calpain-1, calpain-2 and autophagy-related proteins in ARPE-19 cells; (E,F) Confocal images of ARPE-19 cells labelled with anti-LC3 (red) and nuclear-stained with DAPI (blue). Scale bar = 20 μm. ## p < 0.01, ### p < 0.001 compared with control. * p < 0.05, ** p < 0.01, compared with EBSS group.