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Phylogenetic tree of the S40 gene family in the sequenced genome Brassicaceae family including Arabidopsis thaliana, Brassica napus, Brassica oleracea, and Brassica rapa. Red dot indicates the S40 senescence reference gene, AtS40-3. The MEGA 6.0 program was employed to construct a neighbor-joining phylogenetic tree using the JTT model with 1000 bootstrap replicates. The full-length amino acid sequences were aligned using Clustal Omega.
