Figure 1.

Genomic organization of the RASSF1 gene. 12 kb of the genomic region of chromosome 3p21.3 (hg19: 50,379,000–50,367,000) is shown. Exons are numbered and depicted as black and blue boxes indicating coding and untranslated regions, respectively. The RASSF1A isoform is transcribed from a CpG island containing 84 CpG sites, that is often hypermethylated in cancer. Transcription of RASSF1C initiates a downstream located CpG island consisting of 139 CpGs. Transcription start sites are indicated with arrows and transcription end sites are marked with 3′. ZMYND10 represents the last exon (12) of the zinc finger MYND-type containing 10 gene, which is located 200 bp upstream of RASSF1A promoter.