Figure 1.

Northern hybridization analysis of OsY37 gene transcript accumulation during leaf senescence. Rice cv. “Akitakomachi” top leaves were harvested at 47 d before heading (a) and flag leaves after heading dates (b). Total RNA was extracted from the leaves of several plants at the same growth stages. RNA (20 µg) was separated by agarose/formaldehyde gel electrophoresis, blotted onto a nylon membrane, and allowed to hybridize with the [α-³²P]-dCTP-labeled 251 bp OsY37^I segment (Figure S2). Thick and thin arrows point to 1 and 3–4 kb transcripts, respectively. The same membrane was re-hybridized with cDNA of Arabidopsis thaliana 18S rRNA as a loading control, which is shown below the OsY37^I segment-probed hybridization. After hybridization, the membrane was washed under high stringency washing conditions.