Figure 7.

Transcriptional gene expression determined by reverse transcription polymerase chain reaction (RT-PCR) analysis. Total RNA was extracted from flag leaves at the indicated days after heading. Semi-quantititative RT-PCR was performed using OsY37 primers Osy2 and Osy752 which give rise to the amplification of 754 DNA fragment (a–d). To amplify OsY37SRDX transcript, OsY37-specific forward primer; OsyPri1 and SRDX-specific reverse primer; SRDX-R were used. Actin gene expression analysis was carried out similarly with forward; OsActinF318 and reverse; OsActinR675 primers for internal standard. Wild-type and OE lines of Nipponbare (a); Kinmaze (b); RNAi lines of Nipponbare (c); Kinmaze (d); and SRDX lines of Nipponbare (e). Names of plant lines are shown in Figure 4.