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. 2017 Oct 23;18(10):2218. doi: 10.3390/ijms18102218

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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RGO (A) and RGW (B) protected Aβ_25–35-stimulated TNF-α production. PC12 cells were treated with Aβ_25–35 for 30 h and then cellular lysates were analyzed by Western blot assay. Cells were pretreated with RGO or RGW for 1 h, then stimulated with 50 μM Aβ_25–35. The culture medium samples were collected 24 h later for NO and PGE₂ assay. Effect of RGO and RGW on production of NO (C) and PGE₂ (D). Effect of RGO on expression of iNOS (E) and COX-2 (G). Effect of RGW on expression of iNOS (F) and COX-2 (H). PC12 cells were treated with Aβ_25–35 for 30 h and then cellular lysates were analyzed by Western blot assay. The separated proteins were analyzed by Western blot, Griess and ELISA assay as described in Materials and Methods. ### p < 0.001 compared to control group; *** p < 0.001 and ** p < 0.01 compared to Aβ_25–35 alone (ANOVA followed by Duncan’s multiple comparison test).

RGO (A) and RGW (B) protected Aβ_25–35-stimulated TNF-α production. PC12 cells were treated with Aβ_25–35 for 30 h and then cellular lysates were analyzed by Western blot assay. Cells were pretreated with RGO or RGW for 1 h, then stimulated with 50 μM Aβ_25–35. The culture medium samples were collected 24 h later for NO and PGE₂ assay. Effect of RGO and RGW on production of NO (C) and PGE₂ (D). Effect of RGO on expression of iNOS (E) and COX-2 (G). Effect of RGW on expression of iNOS (F) and COX-2 (H). PC12 cells were treated with Aβ_25–35 for 30 h and then cellular lysates were analyzed by Western blot assay. The separated proteins were analyzed by Western blot, Griess and ELISA assay as described in Materials and Methods. ### p < 0.001 compared to control group; *** p < 0.001 and ** p < 0.01 compared to Aβ_25–35 alone (ANOVA followed by Duncan’s multiple comparison test).

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