Figure 3.

Evaluation of the effect of argan oil on 7-ketocholesterol-induced plasma membrane permeability in 158N murine oligodendrocytes by flow cytometry after staining with propidium iodide. After 24 h of culture, 158N murine oligodendrocytes were cultured for an additional 24 h without or with 7KC (25 µM) in the absence or presence of argan oils (Agadir or Berkane; Morocco; 0.1% v/v) or of α-tocopherol (400 µM) used as the positive control. Argan oils and α-tocopherol were added to the culture medium 2 h before 7KC. The cytoprotective effect of argan oils on 7KC-induced plasma membrane permeability was evaluated by flow cytometry after staining with propidium iodide (PI). Plasma membrane permeability was determined by the percentage of PI positive cells. The experiments were carried out twice in triplicate. Data are mean ± SD of two independent experiments carried out in triplicate. The significance of the relationship between vehicle and cells treated with 7KC, argan oils or α-tocopherol was calculated by the Anova test (Sidak’s multiple comparisons); * p ≤ 0.05.The significance of the relationship between cells treated with 7KC alone, 7KC and argan oils cotreatment, or 7KC and α-tocopherol cotreatment was calculated by the Anova test (Sidak’s multiple comparisons); ^# p ≤ 0.05. No significant difference was observed between control and vehicle-treated cells (EtOH 0.95% v/v).