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. 2017 Oct 23;18(10):2220. doi: 10.3390/ijms18102220

Figure 6.

Figure 6

Effects of argan oil on 7-ketocholesterol-induced decreased transcription of PPARα. After 24 h of culture, 158N murine oligodendrocytes were cultured for an additional 24 h without or with 7KC (25 µM) in the absence or presence of argan oils (Agadir or Berkane; Morocco; 0.1% v/v) or of α-tocopherol (400 µM) used as the positive control. Argan oils and α-tocopherol were added to the culture medium 2 h before 7KC. The mRNAs of PPARα was quantified by RT-qPCR. 36B4 was used as the reference gene. Data are mean ± SD of two independent experiments carried out in triplicate. The significance of the relationship between vehicle and cells treated with 7KC, argan oils or α-tocopherol was calculated by the Anova test (Sidak’s multiple comparisons); * p ≤ 0.05. The significance of the relationship between cells treated with 7KC alone, 7KC and argan oils cotreatment, or 7KC and α-tocopherol cotreatment was calculated by the Anova test (Sidak’s multiple comparisons); # p ≤ 0.05. No difference was observed between control and vehicle (EtOH 0.95%)-treated cells.