Figure 3.

PKC pathway is activated by intermittent PTH (1–34). Protein kinase activity of PKC (A) and PKA (B) in human mesenchymal stem cells at day 7 of osteogenic differentiation after intermittent (I-0.2 nM) treatment for 2 h per day for 7 days or continuous (C-0.2 nM) treatment for 2 days for 7 days with 0.2 nM PTH (1–34). Relative kinase activity was normalized to the concentration of crude protein and compared to that in the 0-nM group. Data are represented as mean ± SD (n = 3). Statistical data analysis was performed by performing one-way ANOVA with Tukey’s post-hoc tests. Different letters represent significant differences between groups; those with the same letters were not significant (p < 0.05).