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. 2017 Oct 24;18(10):2228. doi: 10.3390/ijms18102228

Figure 2.

Figure 2

Figure 2

Inhibitory effect of coumestrol on anchorage-independent cell growth of lung cancer cells (a) and colon cancer cells (b,c). The detailed procedure is described in the Materials and Methods. Briefly, the colony formation of the cancer cells was tested with 0.3% agar matrix as the top layer with 0.6% agar matrix as the base layer. The colony formation was captured with the X 40 magnification. Data are representative of three independent experiments, which gave similar results. The asterisks (***) indicate a significant difference of p < 0.001 compared to the untreated group.