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. 2017 Aug 29;7(15):3715–3731. doi: 10.7150/thno.19678

Figure 1.

Figure 1

Identification of the placental-homing peptide NKGLRNK [A] Mouse placental tissue was incubated with a T7 bacteriophage library and bound phages were titered, amplified and purified. Phage titer (pfu/mg tissue) was measured using a plaque-forming assay (n=3). [B] Following 3 rounds of ex vivo selection, the resulting phage pool was injected into the tail vein of a pregnant mouse. Placentas were harvested and bound phage pools were titered, amplified and purified. Four rounds of in vivo screening were performed in total; phage titer is expressed as pfu/mg tissue (n = 3). [C-E] NKGLRNK labelled with 5(6)-carboxyfluorescein (FAM; 200 μg) was injected into the tail vein of pregnant mice (n = 5; E10.5 - E17.5). Following cardiac perfusion to remove unbound peptide, placentas were harvested and assessed by fluorescence microscopy. Representative images are shown. FAM-labelled peptides, green; DAPI (nuclei), blue. JZ = junctional zone; Lab = labyrinth; SA = spiral artery.