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. 2017 Aug 29;7(15):3715–3731. doi: 10.7150/thno.19678

Figure 5.

Figure 5

Targeted delivery of SE175 reduces expression of the oxidative stress markers 4-HNE, COX-1 and COX-2 in eNOS-/- mice Immunostaining of the mouse placental labyrinth with antibodies to [A - F] 4-HNE; [G - L] COX-1; [M - R] COX-2. [A, G, M] PBS-treated C57BL/6J mice, [B, H, N] PBS-treated eNOS-/- mice; [C, I, O] eNOS-/- mice treated with CNKGLRNK-decorated liposomes containing PBS (L-PBS); [D, J, P] eNOS-/- mice treated with CNKGLRNK-decorated liposomes containing SE175 (L-SE175); [E, K, Q] eNOS-/- mice treated with free SE175; [F, L, R] eNOS-/- mice, IgG control. Blue = hematoxylin; Brown = DAB; scale bars = 50 µm. n = 3 placentas from N = 4 mice were assessed; representative images are shown. [S-U] Quantitative assessment of DAB:Hematoxylin ratio of total area of [S] HNE; [T] COX-1; [U] COX-2. Mean ± SEM. Horizontal red dashed line represents vehicle control mean. Means were compared using a Kruskal Wallis test with Dunnett's post hoc test. *P<0.05; **P<0.01.