Figure 6.

SE175 does not alter human placental growth or function [A] First trimester and [B] term human placental explants were incubated with Rh-CNKGLRNK (0.27 µmol/L) for up to 3 h. Peptide binding and uptake were assessed by fluorescence microscopy; (n = 3). Representative images are shown. Rh-labelled peptides, red; DAPI (nuclei), blue. ST = syncytium; VS = villous stroma; scale bar = 50 µm. [C-F] Term placental explants were treated with DMSO (vehicle control), SE175 or the nitric oxide donor SNAP for 24h. [C] Na⁺-dependent [¹⁴C]MeAIB uptake at 30, 60 and 90 min; mean ±SEM; n = 6. [D] Percent control hCG secretion as measured by ELISA; mean, n = 6. [E] Percent of Ki67-positive (in-cycle) cells; mean, n = 5. [F] Percent of M30-positive (apoptotic) cells; mean, n = 5.