Figure 5.

The protective role of AP against H₂O₂-induced endothelial apoptosis. A) The apoptosis of endothelial cells was labelled with annexin V-FITC/propidium iodide (PI) and determined using flow cytometry. EA.hy926 cells were seeded on 12-well plates overnight and then pre-incubated with AP (25 μM) for 15 min, followed by stimulation with H₂O₂ (200 μM, 2 h or 4 h) in HBSS medium. B) The representative blots for phosphorylation of JNK, ERK and p38 in the presence of AP probe upon H₂O₂ (200 μM, 12 h) exposure in DMEM medium. Summary of phospho-JNK (C), phospho-ERK (D) and phospho-p38 (E) were indicated as densitometric values. **p < 0.01.