Figure 4.

miR-194 abolished cell migratory activity. The effect of stable transfection of miR-194 in HCT116 cells was investigated. (A) HCT116^ZsGreen and HCT116^{ZsGreen-miR-194} cells were seeded into transwell culture plates. The migrated cells were stained and calculated from ten randomly selected fields per well. Three independent wells were averaged and shown with SD. * P < 0.05. (B) The effect of stable transfection of miR-194 on EMT markers was measured at the mRNA level. Data was normalized to the expression level of GAPDH and compared to HCT^116ZsGreen using the ddCt method. (C) The effect of stable transfection of miR-194 on EMT markers was measured at the protein level. β-Actin was used as an internal control. (D) β-CATENIN (red) or (E) F-actin (Rhodamine phalloidin, red) and nucleus (DAPI, blue) were monitored by fluorescence microscopy. Bar indicates 100 μm.