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. 2017 Sep 15;7(16):3972–3988. doi: 10.7150/thno.18990

Figure 6.

Figure 6

IL11 and IL15 derived from CAFs synergistically activate STAT3 signaling in endothelial cells. (A-C) The total and phosphorylated proteins of STAT3, VEGFR2, PI3K/AKT, ERK and P38/MAPK in HUVECs cells were determined by Western blotting. β-Actin is the loading control. Levels of p-STAT3 (B) and p-VEGFR2 (C) were quantified as relative pixel intensity to β-Actin. (D-F) Cell invasion (D) and tubule formation (E,F) abilities of HUVECs were tested in the presence of CM from IL11 and IL15 overexpressing NFs with Axitinib, S3I-201 (E) or neutralizing anti-VEGF antibody (F); Scale bar, 300 μm. (n=3; NS, not significant; *P<0.05; **P<0.01).