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. 2017 Nov 2;48:72. doi: 10.1186/s13567-017-0476-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Morphologic changes in DCs after stimulation with B. canis or its purified LPS. Scanning electron microscopy analysis of human or canine DCs stimulated for 24 h with live B. canis or it purified LPS. Human B. canis-stimulated DCs (A) developed numerous long projections and their cell size was at least five times greater than non-stimulated DCs (D). LPS-stimulated DCs (B, C) also increased cell size and developed projection in a less intense manner. Canine stimulated DCs (E–G) also showed increased cell size and developed projections. Non-stimulated human (D) and canine (H) DCs were round shaped with short projections.