Fig. 4.

Protein expressions of Pdx1, NeuroD1 and p-NeuroD1 in the F2 offspring islets at the age of 12 weeks. a Protein expression levels of Pdx1, NeuroD1 and p-Neuro D1 by western blot analysis; (b) the relative protein levels in (a). The western blot analyses showed that protein levels of Pdx1 and p-NeuroD1 in pancreatic islets of the F2-HF group were significantly lower than those of the F2-SC group at the age of 12 weeks. No significant difference in protein expression of NeuroD1 was found between these two groups. *, P < 0.05 versus F2-SC. N = 3 per group