Fig. 4. CD spectra of the G segments and six different split modes. Strands added in each sample in (a), (b) and (d) have been indicated in the figure. The relative reference CD values for different split modes in (c) and (e) have also been indicated in the figure. (f) CD melting curves for four different DNA complexes (S + Gx + Gy) at 276 nm in lithium cacodylate buffer. Concentrations of the DNA strands were 10 μM in (a), 5.0 μM in (b) and (d) and 2.0 μM in (f).